Screening individual hybridomas by microengraving to discover monoclonal antibodies
نویسندگان
چکیده
منابع مشابه
Monoclonal Antibodies
SUMMARY Monoclonal Antibodies are identical antibodies and Can be generated in ultimated amounts by countinuos cultures of single antibody- secreting cells. These cell lines are produced by cell fusion of normal lymphocytes to cells of a myeloma tumor cell line, which confers on the antibody producing hybrid cell, immortality and the ability grow as atumor in animals. monoclonal antibodies c...
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We report the establishment of human-human hybridomas producing monoclonal antibody of predefined antigenic specificity. The U-266 human myeloma cell line was incubated in the presence of 8-azaguanine, and a rapidly growing, 8-azaguanine-resistant, hypoxanthine/amethopterin/thymidine (HAT) medium-sensitive mutant line, U-266AR1, was selected. These cells were fused with lymphoid cells from unin...
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Alpha-fetoprotein (AFP), a serum glycoprotein belonging to the onco-developmental proteins group, serves as a marker both in cancer research and in studies concerning fetal development and fetal pathophysiology. Monoclonal anti-AFP antibodies are essential reagents in developing appropriate techniques for measurement of this protein. In this study, in order to produce anti-AFP monoclonal a...
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Using the human lymphoblastoid cell line, GM 4672, and PBL of Gambian adults immune to Plasmodium falciparum (Pf) malaria, we have produced human-human hybridomas and selected those that produce mAb against Pf antigens. The fusion frequency, using PWM-stimulated donor lymphocytes was between 6.8 X 10(-5) and 1.5 X 10(-6). Using immune fluorescence, immune precipitation, and Pf in vitro growth i...
متن کاملIndividual differences in lutropin immunoreactivity revealed by monoclonal antibodies.
We screened 11 monoclonal anti-lutropin (LH) antibodies for use in two-site immunometric combinations, with time-resolved fluorescence as the detection principle. Seven immunofluorometric assays (IFMA), six detecting only the intact hormone and one detecting both the intact hormone and free beta subunit, were compared with each other and with a conventional radioimmunoassay involving a polyclon...
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ژورنال
عنوان ژورنال: Nature Protocols
سال: 2009
ISSN: 1754-2189,1750-2799
DOI: 10.1038/nprot.2009.40